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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Mol Cell Neurosci. 2007 Dec 15;37(4):696–707. doi: 10.1016/j.mcn.2007.12.015

Figure 4.

Figure 4

Hypothalamic Pitx2cre/null neurons are shifted medially and away from the floor plate. β-galactosidase positive cells were counted in three different transverse sections from Pitx2cre/+; N-lacZ (A) and Pitx2cre/null; N-lacZ (C) littermate embryos at the level of the hypothalamus (same sections as in figure 7 I, M). β-galactosidase positive cells were more abundant in medial regions of the hypothalamus toward the third ventricle (IIIv) of Pitx2cre/null; N-lacZ embryos compared to Pitx2cre/null; N-lacZ embryos. The overall number of β-galactosidase positive cells was slightly increased in the mutant (N = 590 cells for Pitx2cre/+; N = 746 cells for Pitx2cre/null). There was also a minimal shift of βgal+ cells away from the floor plate (fp) in Pitx2cre/null; N-lacZ cells compared to Pitx2cre/+; N-lacZ embryos (C, D). Scale bar = 100 μm.