Table 1.

Primer sequences of genes used for mRNA quantification

Protein	NCBI Accession #	Forward Primer (5′ to 3′)	Reverse Primer (5′ to 3′)
ACC-α	NM_133360	CAGGGACTATGTCCTGAAGCA	GGTCATGTGGACGATGGAGT
AOX	NM_015729	TTATGCGCAGACAGAGATGG	TATGTGGCAGTGGTTTCCAA
CPT1A	NM_013495	CAGCAGCAGGTGGAACTGT	GGAAACACCATAGCCGTCAT
CYP2E1	NM_021282	CCAAAGAGAGGCACACTTCC	GCACAGCCAATCAGAAAGGT
FAS	NM_007988	GGCATCATTGGGCACTCCTT	GCTGCAAGCACAGCCTCTCT
mtHMGS	NM_008256	CCTACCGCAAGAAGATCCAG	GAAAGGCTGGTTGTTTCCAG
MCAD	NM_007382	TGATCAACGCGCACATTC	GAACGTTCCCAGGCCAAG
MTTP	NM_008642	GGATCCTCTTCTGCCTATACTGG	TGTCAAGGCTGTATGTGGAC
SCD-1	NM_009127	CCTCCGGAAATGAACGAGAG	CAGGACGGATGTCTTCTTCCA
TBP	NM_013684	ACTTCGTGCAAGAAATGCTGAA	TGTCCGTGGCTCTCTTATTCTCA

The abbreviations used are: ACC-α, acyl-CoA carboxylase-alpha; AOX, acyl-CoA oxidase; CPT1A, carnitine palmitoyl transferase 1A; CYP2E1, cytochrome P450 2E1; FAS, fatty acid synthase complex; mtHMGS, hydroxy-methyl-glutaryl CoA synthase (mitochondrial form); MCAD, medium chain acyl-CoA dehydrogenase; MTTP, microsomal triacylglycerol transfer protein; SCD-1, stearoyl CoA desaturase-1; TBP, TATA box-binding protein.