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. 2008 May 21;3(5):e2237. doi: 10.1371/journal.pone.0002237

Figure 3. RNA binding by M. tuberculosis ThyA and ThyX.

Figure 3

M. tuberculosis thymidylate synthases do not bind to their cognate mRNA. 0.35 nM of 32P labeled M. tuberculosis ThyX mRNA (lanes 1 and 2), M. tuberculosis ThyA mRNA (lanes 3 and 4), or P. falciparum DHFR-TS mRNA (lanes 5 and 6) was incubated in the presence or absence (lanes 1, 3, and 5) of purified M. tuberculosis ThyX (3.5 µM, lane 2), M. tuberculosis ThyA (3.5 µM, lane 4), or P. falciparum DHFR-TS (500 nM, lane 6). Unbound RNA was digested with 3 units of RNase T1 prior to electrophoresis [22].