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. 2008 May 21;3(5):e2237. doi: 10.1371/journal.pone.0002237

Figure 5. Steady-state kinetics of M. tuberculosis ThyA.

Figure 5

(A) Titrations of mTHF (29, 143, 571, 1143 µM) with varying concentrations of dUMP (•, 1.875 µM; ▾, 3.75 µM; ▪, 7.5 µM; ♦, 15 µM). (B) Double reciprocal plot of data from plot A showing a sequential reaction mechanism. (C) Titrations of dUMP (1.875 µM, 3.75 µM, 7.5 µM, 15 µM) with varying concentrations of mTHF (•, 29 µM; ▾, 143 µM; ▪, 571 µM; ♦, 1143 µM). (D) Double reciprocal plot of data from plot C showing a sequential reaction mechanism. Lines in A and C are plots of Michaelis-Menten equations with calculated constants. Lines in B and D are linear regressions. Error bars are propagation of standard deviations.