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. 1997 Jul 8;94(14):7577–7582. doi: 10.1073/pnas.94.14.7577

Figure 2.

Figure 2

(A) Restriction endonuclease map and sequencing strategy for pSHDM(116-10C) cDNA. Lines with arrowheads indicate direction and extent of sequencing. A predicted open reading frame is boxed. (B) Nucleotide sequence of SCGF cDNA and deduced amino acid sequence. pSHDM(116-10C) cDNA was sequenced using sets of oligonucleotide sequencing primers and dideoxy terminator cycle sequencing AmpliTaq on an Applied Biosystems model 373A sequencer. Numbering is from the 5′ end of the DNA sequence and from initiation methionine of the amino acid sequence. The putative signal peptide sequence is underlined. Cysteine residues are boxed. The stop codon is indicated by asterisks. Poly(A) signal is dot-underlined in the 3′-untranslated region. (C) Hydrophobicity of SCGF amino acid sequence. Each plot was calculated by the method of Hopp and Wood (22) using the dnasis Mac program (Hitachi, Tokyo).