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. Author manuscript; available in PMC: 2008 May 15.
Published in final edited form as: Methods Enzymol. 2008;438:367–389. doi: 10.1016/S0076-6879(07)38026-9

Fig 2.

Fig 2

Intracellular localization of Ras proteins in immortalized fibroblasts. Rce1 flx/flx cells express normal levels of Rce1, whereas Rce1 α/α fibroblasts are completely deficient in Rce1 expression. Cre adenovirus fully converted the Rce1flx/flx fibroblasts into Rce1 α/α fibroblasts. Fibroblasts were fractionated into cytosolic (S100) and membrane (P100) fractions; Ras proteins were immunoprecipitated and analyzed on western blots of sodium dodecyl sulfate-polyacrylamide gels with antibody Ab-4. Note higher molecular weight of the Ras proteins in Rce1 α/α fibroblasts. Reproduced, with permission from Molecular and Cellular Biology (Bergo et al., 2002a).