Fig 3.

Southern blot assessment of the ratio of Rce1^flx and Rce1^α alleles during the growth of mixed cultures of Rce1^flx/flx and Rce1^α/α fibroblasts. Mixed cultures of Rce1 ^flx/flx and Rce1^α/α cells were obtained by infecting immortalized Rce1^flx/flx fibroblasts with lacZ adenovirus and Cre adenovirus. Southern blots showing the ratio of Rce1^α and Rce1^flx alleles at different passages in four independent experiments. These experiments show that the Rce1^flx/flx cells, which express Rce1, grow more rapidly than the Rce1^α/α fibroblasts, which lack Rce1 expression. Reproduced, with permission from Molecular and Cellular Biology (Bergo et al., 2002a).