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. 2008 May 28;3(5):e2321. doi: 10.1371/journal.pone.0002321

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Tao, Hancock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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CD4+CD25− and CD4+CD25+ were isolated by MACS separation and stimulated in vitro with PMA/ionomycin plus IL-2 for 0, 24, 48 and 72 h (IL-2 alone as no stimulation control). T cell apoptosis at the indicated time points was detected by Annexin V staining and flow cytometry analysis. Histogram data with the figure in each gated population indicating percentage of Annexin V+ cells in the total (left panels) or live (right panels) cell populations. Data are representative of 3 experiments with similar results.