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. 2008 Apr 24;8:45. doi: 10.1186/1471-213X-8-45

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Copyright © 2008 Masui et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nuclear localization of Rex1. The expression vectors of EGFP-tagged or HA-tagged Rex1 were transiently transfected into HeLa and ES cells, and the localization of these transgene products were detected by fluorescent microscopy directly (for EGFPRex1) or after immunostaining for HA-tag (for HARex1). Phase contrast (left), fluorescent (middle) and their merged image (right) were shown for each transfectants. The fluorescent signals were localized in nuclei in both HeLa and ES cells for both chimeric Rex1 proteins.