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Activated HER-2 up-regulates survivin expression. Three pTRE-HER2-transfected (H1, H2 and H3) and two pTRE-transfected control (C1 and C2) MCF-7 clones were incubated with or without doxycycline. Whole cell protein extracts were prepared from untreated cells or cells treated with doxycycline for 48 hours. Extracts (25 ug protein/lane) were analyzed by Western blotting for the expression of survivin and phosphorylated HER-2).
