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Analysis of total ERK 1/2, STAT 3, AKT 1 and their phosphorylated forms in HER2-overexpressing and control cells. pTRE-HER2 and pTRE-transfected MCF-7 clones were incubated with or without doxycycline for 48 hours. Whole cell extracts were prepared and analyzed by Western blotting with the indicated antibodies. Experiments were repeated twice with independently collected extracts.
