Figure 7.

Signaling pathways involved in survivin regulation in HER2 over expressing MCF7cells. A- HER2 induced MCF7 cells were incubated for 48 hours with specific inhibitors of PI3K (10 μM LY294002) AKT1/2/3 (15 μM AKT IV Inhibitor) and ERK1/2 (10 μM U0126) or without inhibitor (control) and analyzed by western blotting for expression of survivin. B- To confirm the inhibition of AKT and PI3K, HER2 induced MCF7 cells were incubated for 48 hours with specific inhibitors of PI3K (10 μM LY294002) AKT1/2/3 (15 μM AKT IV Inhibitor). ERK1/2 (10 μM U0126) and without inhibitor MCF-7 cells were used as controls, and analyzed by western blotting for expression of phospho-ribosomal protein S6 (S235).