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. 2008 Apr 29;9:197. doi: 10.1186/1471-2164-9-197

Table 2.

Quantitative RT-PCR validation of 8 selected genes.

Gene Symbol Microarrays Real-time PCR

FC P-value FC P-value
Kif5b 12.17 < 0.001 3.14 0.005
Ptger2 4.58 < 0.001 3.42 0.01
Dkkl1 -3.76 < 0.001 -10.01 0.006
Grb10 -2.68 < 0.001 -10.32 0.09
Tnc -1.76 0.001 -4.16 0.06
Wnt4 1.13 0.229 1.82 0.047
cyclin D1 1.08 0.66 1.95 0.12
cmyc 0.85 0.08 1.02 0.97

Comparison between array experiment and real-time PCR for 8 selected genes is shown. Log-fold change in real-time PCR was calculated from the difference in normalised expression between the CBA and QSi5 strains of mice. P-value was calculated from 2-tailed Student's t-test.