Table 2.
Quantitative RT-PCR validation of 8 selected genes.
Gene Symbol | Microarrays | Real-time PCR | ||
FC | P-value | FC | P-value | |
Kif5b | 12.17 | < 0.001 | 3.14 | 0.005 |
Ptger2 | 4.58 | < 0.001 | 3.42 | 0.01 |
Dkkl1 | -3.76 | < 0.001 | -10.01 | 0.006 |
Grb10 | -2.68 | < 0.001 | -10.32 | 0.09 |
Tnc | -1.76 | 0.001 | -4.16 | 0.06 |
Wnt4 | 1.13 | 0.229 | 1.82 | 0.047 |
cyclin D1 | 1.08 | 0.66 | 1.95 | 0.12 |
cmyc | 0.85 | 0.08 | 1.02 | 0.97 |
Comparison between array experiment and real-time PCR for 8 selected genes is shown. Log-fold change in real-time PCR was calculated from the difference in normalised expression between the CBA and QSi5 strains of mice. P-value was calculated from 2-tailed Student's t-test.