Table 1.
Sequences generated by Pan-retrovirus RT-PCR of density gradient fractions containing the peak of RT activity or the corresponding control fraction
| Culture | MSRV-cpol | ERV9 (v) | Artefacts (vi) | Total clones |
|---|---|---|---|---|
| LM7P (i) | 16 | 4 | 6 | 26 |
| PLI-1 (ii) | 9 | 1 | 13 | 23 |
| MS B cell line (iii) | 9 | 2 | 8 | 19 |
| Control B cell line (iv) | 0 | 0 | 26 | 26 |
i, LM7-infected choroid plexus cell culture; ii, MS patient-derived choroid plexus cell culture (PLI-1); iii, MS patient-derived spontaneous B cell line (immortalized by endogenous Epstein–Barr virus strain); iv, non-MS control B cell line; v, clones with >90% homology with the GenBank sequence HSERV9 are designated ERV9 in this study; vi, PCR artefacts including primer multimers, concatemers, single primer amplifications, etc.