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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biomaterials. 2008 Mar 14;29(17):2646–2655. doi: 10.1016/j.biomaterials.2008.02.019

Figure 2.

Figure 2

Automated image analysis using ImageJ (NIH). (a) Original fluorescence micrograph of endothelial cells subjected to stretch at 5 Hz for 12 hrs and stained with calcein AM. During processing, images were thresholded to create a binary black and white image (b) and ellipses were automatically fitted around each cell (c). The orientation angle (θ) was calculated as the angle between the major axis of the ellipse and a line extending from the center of the well to the ellipse centroid (d). (e) Alignment histogram with the distribution of cell orientation angles.