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. 2008 Jun;10(6):549–562. doi: 10.1593/neo.08286

Figure 3.

Figure 3

Tumor-associated IL-1, matrix adhesion, migration, and angiogenesis induction. (A–G) Three wt, IL-1α-/-, IL-1β-/-, and IL-1αβ-/- fibrosarcoma lines were tested for the following: (A) Adhesion molecule expression by flow cytometry. The percentage of stained cells and the mean intensity of staining are shown. (B) Adhesion to components of the extracellular matrix. Adhesion was measured after a 2-hour incubation by crystal violet staining. (C) Transwell migration, evaluated after a 4-hour incubation by crystal violet staining. (D) Migration into a wounded monolayer 48 hours after wounding. A representative example of one wt, IL-1α-/-, IL-1β-/-, and IL-1αβ-/- fibrosarcoma line is shown. (E) Cable formation on matrigel evaluated after 24 hours. (F) uPAR expression was evaluated by flow cytometry. The percentage of stained cells and the mean intensity of staining are shown. (G) MMP2 and MMP9 expression in 3 wt, IL-1α-/-, IL-1β-/-, and IL-1αβ-/- fibrosarcoma lines as revealed by zymography. (A–C and F) Mean ± SD of three distinct lines are presented, and significant differences between wt, IL-1α-/-, IL-1β-/-, and IL-1αβ-/- lines are indicated by an asterisk. All experiments were repeated at least three times and revealed comparable results.