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Overview of the TIMP-3 amplicon locations. Map showing the amplicons used in this study in relation to the transcription start of the TIMP-3 gene. Whereas the whole CpG island spans more than 1200 bp, the amplicon for the MethyLight assay covers 13 CpG sites more than 155 bp proximal to the coding region. An extended amplicon for bisulfite sequencing covers 48 CpG sites (in total, 442 bp).
