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. 2008 May 28;3(5):e2274. doi: 10.1371/journal.pone.0002274

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Hodge, Stanewsky.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Confocal image of the dorsal projections of the LNv neurons revealed by anti-PDF antibodies. The panels show representative samples of adult brains containing clock neurons with normal levels of Shaw (UAS-Shaw/CyO), elevated levels of Shaw (tim-GAL4/UAS-Shaw), a dominant-negative form of Shaw (tim-GAL4/UAS-ShawTR), or reduced levels of Shaw (tim-GAL4/UAS-Shaw-RNAi) that were fixed at the two time points indicated (ZT9 and ZT21). (B) Quantification of average staining intensity of the dorsal LNv projections from a single experiment (n = 7 to 10 hemispheres). Error bars represent SEM. In control brains PDF accumulation is higher during the day compared to the night. This circadian difference in PDF intensity is not seen when Shaw levels are manipulated in the clock neurons using tim-GAL4. In addition PDF tends to accumulate to higher levels compared to wild-type when Shaw levels are increased, while reduction or dominant-negative forms of Shaw tend to have the opposite effect.