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. 2008 May 28;3(5):e2277. doi: 10.1371/journal.pone.0002277

Figure 4. PML Ib can function in the cellular anti-viral defense against HSV-1.

Figure 4

(A) A schema of the cellular model and infection protocol. (B) B104-1-1 cells were transfected with vector, PML I, or PML Ib. Stable cell clones were selected. PML I and PML Ib cells were immunostained with PG-M3. (C and D). Stable cell clones were infected with 0.01 PFU/mLHSV-1 -F. Infected cells were harvested at the indicated times for Western blot analysis (C), and analysis of viral tier (D). Expression of PML Ib reduced viral protein production in cells infected with HSV-1(F) (C). Protein lysates (10 µg/sample) were separated by electrophoresis and blotted with antibodies against the HSV-1 viral proteins ICP0 and US11. β-Actin was used as loading control. (Results are representative of multiple experiments.) (D) Viral titers at different time points were determined in triplicate by standard plaque assay using VERO cells.