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. Author manuscript; available in PMC: 2009 Apr 25.
Published in final edited form as: Mol Cell. 2008 Apr 25;30(2):227–238. doi: 10.1016/j.molcel.2008.03.015

Figure 5.

Figure 5

Overexpression of the GAT domain alone inhibits HIV-1 particle production. (A) Domain organization of GGAs. VHS, Vps27, Hrs and STAM homology; GAT, GGA and Tom; GAE, γ-adaptin ear homology. (B) HeLa cells were transfected with pNL4-3 along with vectors expressing full-length GGA3 or the indicated GGA domains at a 10:1 DNA ratio. Virus release was determined by radioimmunoprecipitation analysis. (C) Quantitation of virus release efficiency (top panel) normalized to GGA fragment expression levels, determined by quantitative Western blotting (WB) with anti-Myc Ab (lower panel). Data from one representative experiment are shown. (D) HeLa cells transfected with vectors expressing full-length GGA3 or individual GGA domains were fixed and immunostained with anti-Myc Ab (green).