Figure 3.

Effect of AGE-rich extracts from serum of patients with chronic kidney disease (CKD) on cultured human aortic endothelial cells (HAEC). HAEC were incubated with AGE that were isolated from normal individuals (NL) or patients with CKD stage 2, 3, or 5 for 30 min, 24 h, and 48 h (50 μg protein/ml). After stimulation, cells were lysed for Western blot analysis using anti–endothelial nitric oxide synthase (anti-eNOS) antibody and normalized with β-actin. The representative Western blots of three independent experiments are shown in A, and the corresponding quantitative plots are shown in B. *Significantly different from normal; #significantly different from CKD2.