Figure 1.

Activity of murine CCN2 promoter fragments with or without rhTGF-β1 treatment in mProx24 cells. Known consensus motifs in the mouse CCN2 promoter and firefly luciferase reporter minigenes are shown. The genomic fragments corresponding to −483 to −434 bp and −434 to −202 bp within the CCN2 promoter contain positive cis-regulatory elements. The latter fragment that contains the SBE and BEC-1 enhances TGF-β1–induced CCN2 promoter activity rather than its basal activity (p-434CCN2L versus p-202CCN2L). In contrast, the former fragment enhances both basal and TGF-β1–induced CCN2 promoter activities (p-483CCN2L versus p-434CCN2L). The data were obtained from four independent experiments.