Figure 3.

Analysis of positive cis-regulatory elements within the −455- to −434-bp region of the CCN2 promoter. Deletion of the −483- to −455-bp region in the CCN2 promoter from p-483CCN2L was without effects (p-483CCN2L versus p-455CCN2L). In contrast, mutation or deletion of sequences corresponding to −455 to −434 bp within the CCN2 promoter significantly reduced both basal and TGF-β1–induced CCN2 promoter activities in mProx24 cells (p-455CCN2L versus p-483mPBEL and p-434CCN2L), suggesting that this region contains positive cis-regulatory elements. The plasmid p-483mPBEL contained a mutated consensus motif in the genomic sequence as shown in Table 1. The data were obtained from four independent experiments.