Figure 4.

Nuclear trans-acting factors bind to the −455- to −434-bp CCN2 promoter fragment. (A) Proteins within nuclear extracts prepared from mProx24 cells incubated in the presence or absence of rhTGF-β1 bound to the −455- to −434-bp CCN2 promoter fragment to form a single shifted band in the EMSA. (B and C) The shifted band was abolished when either the 5′- or 3′-most five bases of the sequence were mutated. These results indicated that this trans-factor (complex) was constitutively expressed and that it recognized the whole sequence of this fragment. The data were obtained from four independent experiments in each case.