Figure 6.

(A) Effects of PARP-1 antisense ODN in vitro. Transfection of PARP-1 antisense ODN by electroporation significantly reduced PARP-1 at the mRNA (i) and protein (ii) levels in control mProx24 cells and cells treated with rhTGF-β1. Because PARP-1 antisense ODN did not affect the expression of Smad2/3 or the phosphorylation of Smad3 (ii), the ODN was unlikely to cause nonspecific knockdown of other mRNA. (B) PARP-1 antisense ODN significantly reduced CCN2 mRNA levels in mProx24 cells treated with rhTGF-β1, whereas treatment with a polyribosylation inhibitor PJ34 (3 μM) was without effects. NC, negative control without rhTGF-β1 treatment; AS, antisense ODN; mAS, mutated antisense ODN. The data were obtained from four independent experiments.