Figure 3.

Effects of anti-MPO IgG on L-E cell interactions in wild-type mice after intrascrotal IL-1β or topical KC. Saline (▪), anti-BSA IgG (○), or anti-MPO IgG (□) was administered intra-arterially (18 μg/g body wt) 4 h after intrascrotal IL-1β (12.5 ng) injection. Changes in leukocyte rolling (A), stationary adhesion (C), and migration (E) were measured for 60 min in postcapillary venules (20 to 50 μm). The results for mice that received saline intra-arterially after intrascrotal injection of vehicle (saline containing 0.1% BSA) are also shown (•). Alternatively, KC (3.9 nM) or vehicle was continuously superfused over the cremaster muscle, and changes in rolling (B), stationary adhesion (D), and migration (F) were assessed. Data are means ± SEM (n = 5 for each treatment group). *P < 0.05, **P < 0.01, ***P < 0.001 versus cytokine-stimulated mice that received anti-BSA IgG.