Figure 1.

Structure ensemble and sequence alignment of the apo LARG PDZ. (A) Backbone superimposition of the 20 lowest-energy structures using residues 67–88, 93–121, and 128–147. (B) Ribbon representation of the average, energy-minimized structure. The βB/βC loop (89–92) and βE/αB loop (122–126) with unobservable resonances are shown in dashed lines. (C) Sequence alignment of the class V PDZ domains. Two critical positions, the first of which immediately follows the βB, and the second of which is at the beginning of the αB, are colored gray and marked below with a triangle (▲). Positions of the mutations are marked above with stars (★).