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Histological section pretreatments
| Pretreatment | Abcam8212 (proteolysis) | Ptf1a dilution | Detection | |
|---|---|---|---|---|
| (M) Embryonic cryosections | None | No | 1:5000 | Cy3 |
| (M) Adult cryosections | None | 5 min RT | 1:3000 | Cy3 |
| (M) Embryonic paraffin | TEG | 3 min RT | 1:6000 | Cy3 |
| (R) Adult cryosections | Citrate | 5 min RT | 1:12,000 | TSA-cy3 |
| (R) Embryonic cryosections | Citrate | No | 1:6000 | TSA-cy3 |
| (H) Adult paraffin | TEG | No | 1:10,000 | TSA-cy3 |
(M), mouse; (R), rat; (H), human; RT, room temperature.
