Fig. 3.

Over expression of HSP70 prevents the suppression of cell growth and induction of cell hypertrophy by FIR. (a) Real-time RT-PCR of HSP70 expression. Cells overexpressing HSP70 were established. (b) Representative Western blots demonstrating increased expression of HSP70 in stably transfected A431 and HSC3 cells. Lane 1, wild-type cells; lane 2, cells transfected with empty pcDNA3.1(+); lane 3, cells transfected pcDNA3.1-HSP70A. (c) Cell proliferation of empty vector-transfected and HSP70-overexpressing cells. Cell proliferation was the same in FIR-irradiated and untreated A431 cells overexpressing HSP70. In contrast, in HSC3 cells, over expression of HSP70 prevented FIR inhibition of cell proliferation. (d) BrdU incorporation in wild-type, empty vector-transfected, and HSP70-overexpressing cells treated with FIR. (e) Hematoxylin and eosin staining of wild-type, empty vector-transfected, and HSP70A-overexpressing A431 and HSC3 cells after 5 days of transfection. Note that the morphology of HSP70A-transfected HSC3 cells was unaffected by FIR irradiation