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In situ mRNA hybridization (using radiolabeled oligoprobes) for hIAPP (a), (b), and GLUT-2 (c), (d) in islets of nontransgenic (a), (c), and hIAPP transgenic (b), (d) female mice after 14 months on high-fat diet. Note that hIAPP mRNA expression is absent in the nontransgenic islet, and that GLUT-2 mRNA expression is reduced in the transgenic islet. Scale bar = 30 μm.
