Fig. 4.

Representative thin-layer chromatographs from wild-type, double-transgenic, and triple-mutant mice. The plate was developed with the solvent system of chloroform-methanol-0.2%CaCl₂; 55:45:10; v/v/v). Triple-mutant mice lack two of the four major brain gangliosides, and have increased levels of a-series gangliosides GM1 and GD1a, compared with wild-type mice. Differences between double-transgenic and wild-type mice were not significant.