FIGURE 1.

MCP-1-induced angiogenesis is mediated via induction of MCPIP. HUVECs were treated with MCP-1 with or without transfection with MCPIP-specific or nonspecific siRNA for 24 h. Expression of MCPIP was detected by RT-PCR (A), real-time PCR (B), and immunoblot (C) analyses. β-Actin was amplified as an internal control for RT-PCR. D, histogram depicting the average MCPIP expression levels in the examined groups as assessed by immunoblot analysis. E, phase-contrast photomicrographs of HUVECs treated with MCP-1 with or without MCPIP-specific or nonspecific siRNA for 24 h (original magnification ×100), and the quantitative tube formation assay (F). ^*, p < 0.001 for treatment with MCP-1 and with nonspecific siRNA.