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. 2008 May 23;283(21):14182–14189. doi: 10.1074/jbc.M709029200

FIGURE 2.

FIGURE 2.

Dysregulated LPS-induced IL-23p19 gene expression in IL-10–/– compared with WT mice. A, prolonged LPS-induced IL-23p19 mRNA expression in IL-10–/– compared with WT mice. Conventional reverse transcriptase-PCR results from WT or IL-10–/– BMDC after treatment with LPS (5 μg/ml) are shown for the indicated times. PCR products were separated on a 2% agarose gel and stained with GelStar. The results are representative of three independent experiments. B, IL-10 inhibits IL-23p19 protein secretion in BMDC derived from IL-10–/– mice. WT and IL-10–/– BMDC were prestimulated with IL-10 (10 ng/ml) overnight or left untreated. The cells were then stimulated with LPS (5 μg/ml) for 20 h, and the supernatants were collected. IL-23p19 protein secretion was detected using enzyme-linked immunosorbent assay (*, p < 0.05). The results show the means ± S.D. from triplicate cultures and are representative of three independent experiments.