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. 2008 May 23;283(21):14182–14189. doi: 10.1074/jbc.M709029200

FIGURE 5.

FIGURE 5.

LPS-induced Bcl3 expression is impaired in IL-10–/– compared with WT mice. A, impaired LPS-induced Bcl3 expression in IL-10–/– mice. BMDC were isolated from WT or IL-10–/– mice and stimulated with LPS (5 μg/ml) for the indicated times. Total RNA was then extracted, and Bcl-3 mRNA levels were analyzed using real time PCR. The data were normalized using the housekeeping gene GAPDH. The results are the means ± S.D. of triplicate samples from one of three independent experiments. B, Bcl3 expression in IL-10–/– mice can be restored by stimulation with IL-10. BMDC were isolated from IL-10–/– mice and stimulated with IL-10 (10 ng/ml) for 24 h. Total protein was extracted, and 20 μg was subjected to SDS-PAGE followed by immunoblotting with Bcl3 and GAPDH specific antibodies. The results are representative of three independent experiments.