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. 2008 May 23;283(21):14182–14189. doi: 10.1074/jbc.M709029200

FIGURE 8.

FIGURE 8.

Enhanced LPS-induced IL-23p19 expression in Bcl3–/– BMDC. A, IL-23p19 mRNA expression in BMDC derived from WT and Bcl3–/– mice. BMDC were isolated from WT and Bcl3–/– mice and stimulated with LPS (5 μg/ml) for the indicated times. Total RNA was extracted and analyzed by RT-PCR for Bcl3 mRNA expression. The data were normalized using the housekeeping gene GAPDH. The results are means ± S.D. of triplicate samples from one of three independent experiments. B, IL-23p19 and C, IL-12p70 protein secretions in BMDC derived from WT, Bcl3–/– and IL-10–/– mice. BMDC were generated from WT (white bars), Bcl3–/– (gray bars), and IL-10–/– (black bars) mice and stimulated LPS (50 ng/ml or 5 μg/ml) for 20 h, and the supernatants were collected. IL-23p19 and IL-12p70 protein secretion was detected using enzyme-linked immunosorbent assay. (*, p < 0.05). The results are the means ± S.D. of triplicate samples from one of three independent experiments.