FIGURE 9.

Analysis of conformational alterations in prelatent antithrombin by proteolytic susceptibility. The susceptibility of native, prelatent, and latent forms of antithrombin to digestion by catalytic levels of the nontarget proteases, chymotrypsin and thermolysin (serpin/protease weight ratio of 10:1), was monitored as a function of digestion time by SDS-PAGE as described under “Experimental Procedures.”