FIGURE 4.

Association of GLI1ΔN with polysomes. Cytoplasmic extracts from 1 × 10⁷ RMS13 cells lysed in the presence of either cycloheximide (A) or EDTA (B) were subjected to a 15–40% sucrose density gradient centrifugation. Twenty fractions were collected (fraction 1, top/light; fraction 20, bottom/heavy). The percentage of GLI1FL (filled squares) or GLI1ΔN(empty circles) transcripts per fraction, determined by real-time RT-PCR, are shown (lower panels). The isolated RNAs from the individual fractions were also subjected to denaturing 1.2% formaldehyde-agarose gel electrophoresis followed by staining with ethidium bromide (upper panels).