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. 2008 May 23;283(21):14581–14589. doi: 10.1074/jbc.M707733200

FIGURE 5.

FIGURE 5.

Reduced GSH levels in Met-KO hepatocytes increase sensitivity to apoptosis. A, Cre-Ctrl and Met-KO hepatocytes were pretreated with BSO (300 μm) for 1 h before incubation with HGF (40 ng/ml) or NAC (10 mm) for 12 h followed by Jo2 (0.5 μg/ml) treatment for 6 h. Apoptotic index was determined by counting apoptotic cells after propidium iodine staining. Each column represents the mean ± S.E. At least 500 nuclei were counted from duplicate cultures from three independent experiments. *, p < 0.05 against Cre-Ctrl cells treated with Jo2 alone or Jo2 + HGF + BSO; **, p < 0.05 against Met-KO treated with Jo2 alone, Jo2 + HGF, or Jo2 + NAC + BSO. B, electromobility shift assays of NF-κB DNA binding activity was performed using nuclear extracts from untreated (NT) Cre-Ctrl cells and Met-KO cells and Met-KO cells treated with 10 mm NAC, 30 μm SN50, or 100 μm sulfasalazine (S) for 12 h. C, cell viability in Cre-Ctrl and Met-KO hepatocytes treated with 30 μm SN50 for 24 h as determined by crystal violet assay. Mean ± S.E. of three independent experiments are shown. *, p < 0.05 against respective Cre-Ctrl cells.