FIGURE 6.

The time course of CIF production and puncta formation. The average time courses of puncta formation in NG115 cells expressing YFP-STIM1 (puncta) and CIF production (CIF) in Jurkat T-lymphocytes following application of TG. The images of NG115 cells were analyzed using ImageJ program (the particle analysis function) after the convolve filter application. The number of puncta was normalized and plotted against time. Each point shows the means ± S.E. from several experiments. The most sensitive detection of early CIF production was achieved using Jurkat cells (∼200 million cells/each time point, repeated in three different cell preparations).