TABLE 1.
Primers used in this study
| Primer | Sequence 5′ → 3′a |
|---|---|
| pTWIN1SrtAΔN24fwd | GGTGGTCATATGAAACCACATATCGATAATTATCTTCAC |
| pTWIN1SrtAfullrev | GGTGGTTGCTCTTCCGCATTTGACTTCTGTAGCTACAAAGATTTTACG |
| pTWIN1SrtAtruncrev | GGTGGTTGCTCTTCCGCAAGTAATTAATGTTAATTGTTTATCTTTACC |
| SrtA R197A | Fwd: GGTTGGGAAAAAGCTAAAATCTTTGTAGC |
| Rev: GCTACAAAGATTTAGCTTTTTTCCCAAAC | |
| SrtA R197K | Fwd: GGTTGGGAAAAAAAAAAAATCTTTGTAGC |
| Rev: GCTACAAAGATTTTTTTTTTTTCCCAAAC | |
| SrtA G167A | Fwd: CCTACAGATGTAGCAGTTCTAGATGAACAAAAAGG |
| Rev: CCTTTTTGTTCATCTAGAACTGCTACATCTGTAGG | |
| SrtA V168A | Fwd: CCTACAGATGTAGGAGCTCTAGATGAACAAAAAGG |
| Rev: CCTTTTTGTTCATCTAGAGCTCCTACATCTGTAGG | |
| SrtA L169A | Fwd: CCTACAGATGTAGGAGTTGCAGATGAACAAAAAGG |
| Rev: CCTTTTTGTTCATCTGCAACTCCTACATCTGTAGG | |
| SrtA D170A | Fwd: CCTACAGATGTAGGAGTTCTAGCTGAACAAAAAGG |
| Rev: CCTTTTTGTTCAGCTAGAACTCCTACATCTGTAGG | |
| SrtA E171A | Fwd: CCTACAGATGTAGGAGTTCTAGATGCACAAAAAGGTAAAG |
| Rev: CTTTACCTTTTTGTGCATCTAGAACTCCTACATCTGTAGG | |
| SrtA Q172A | Fwd: CCTACAGATGTAGGAGTTCTAGATGAAGCAAAAGGTAAAG |
| Rev: CTTTACCTTTTGCTTCATCTAGAACTCCTACATCTGTAGG |
a
Mutated codons are underlined.