TABLE 4.

Kinetic parameters of SrtA Arg 197 mutants

Enzyme	kcat	Km	kcat/Km	Activity decrease from wt rec (syn)
	s−1	mm	m−1s−1	-fold
wt (rec)	1.10 ± 0.06	8.76 ± 0.78	125 ± 18
R197A	6.28 × 10−4 ± 0.60 × 10−5	4.69 ± 0.12	0.13 ± 0.01	960
R197K	1.90 × 10−3 ± 0.20 × 10−4	10.4 ± 0.19	0.18 ± 0.01	690
wt (syn)	0.67 ± 0.05	11.2 ± 1.4	59.7 ± 11	2.1
R197Cit	0.29 ± 0.01	13.7 ± 1.2	21.1 ± 2.7	5.9 (2.8)