Skip to main content
. 2008 May 23;283(21):14230–14241. doi: 10.1074/jbc.M800061200

FIGURE 9.

FIGURE 9.

TNFα deficiency blunts PTP1B overexpression in adipose tissue of mice with diet-induced obesity (DIO). TNFα+/+ (wild-type, WT) and TNFα–/– mice were fed either a low fat chow diet or a high fat diet from 6 weeks of age for 38 weeks for female mice or 26 weeks for male mice. Body weight (a), random-fed blood glucose (b), and serum insulin (c) were measured. Results are means ± S.E. (n = 6–8 per group). *, p ≤ 0.05 compared with WT chow-fed mice by one-way ANOVA. d and e, PTP1B protein was measured in perigonadal (P), perirenal (R), and subcutaneous (S) adipose tissue of chow-fed mice and mice with DIO. PTP1B was detected by immunoblotting and quantified by densitometry. Results are means ± S.E. of PTP1B levels in perigonadal, subcutaneous, or perirenal adipose tissue depots (n = 5–8 per group for each adipose depot). Because diet or TNFα deficiency altered levels of several internal control proteins, PTP1B levels were measured from equivalent amounts of protein, which were verified by Ponceau S staining of immunoblots. *, p ≤ 0.05 compared with WT chow-fed mice and #, p ≤ 0.05 compared with WT DIO mice. Symbols above bars indicate differences determined by one-way ANOVA. Symbols above lines indicate differences determined by two-way ANOVA, with mouse group and adipose depot being the two factors.