Fig. 6.

Comparison between the activities of native cyt b5 (●) and apo-b5 (○) on the metabolism of (S)-flurbiprofen with a 1:2 ratio of CYP2C9:CPR. (A) rate of (S)-flurbiprofen metabolite formation. (B) rate of H₂O₂ formation. (C) rate of NADPH consumption. (D) calculation of reaction coupling to metabolite formation (metabolite formation rate/NADPH consumption rate). Data points represent the average of duplicate determinations except when error bars are present (triplicate ± standard deviation). Experiments with native or apo-b5 were conducted on different days resulting in product levels being slightly offset.