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. 2008 Jun 4;3(6):e2368. doi: 10.1371/journal.pone.0002368

Figure 1. Assessment of liposome CD40 binding activity.

Figure 1

Fig 1a) ELISA plates were coated with anti-human IgG, blocked with 3% BSA, and then incubated with recombinant murine CD40-human IgG1. After washing, various liposomal preparations containing entrapped pneumococcal type 3 polysaccharide (PS) and/or CD40 mAb at varying concentrations (6 or 20 µg per 0.5 ml of liposomal preparation) were added to the plate in two-fold dilutions. Liposomal binding to CD40-was detected using HRP labelled goat anti-rat IgG. 1b) To assess liposomal binding to cell surface CD40, CD154 transfected (filled histograms) or CD40 transfected (open histograms) L929 cells were stained with liposomal preparations at a 1/10 dilution in FACS buffer. Binding was detected using FITC labelled anti-rat IgG.