Table 3.

Effects of mercuric chloride cross-linking and DTT reduction in the presence of glycine on normalized WT, I229C, A288C, and I229C/A288C GlyRs currents

GlyRα1	Gly	Gly (post-HgCl2)	Gly (post-reduction)	n
Cross-linking (HgCl2) and reduction in maximal glycine
WT	0.94 ± 0.05	0.95 ± 0.10	0.97 ± 0.16	5
I229C	0.95 ± 0.05	0.98 ± 0.12	1.02 ± 0.15	5
A288C	0.94 ± 0.09	1.08 ± 0.27	1.19 ± 0.45	5
I229C/A288C	0.84 ± 0.07	0.29 ± 0.04**	0.84 ± 0.11	5

^**p < 0.01 significantly different from initial glycine response (normalized to 1.00 and not shown) for each receptor by one-way ANOVA and the Dunnett’s post-test. Two applications of maximal glycine were followed by cross-linking with mercuric chloride (10 μmol/L, 1 min), maximal glycine, reduction with DTT (10 mmol/L, 3 min), and a final application of maximal glycine. Washout times were 15 min intervals between applications. Mercuric chloride and DTT were applied in the presence of maximal glycine. Cross-linking with iodine in the presence of glycine resulted in decreased receptor responses in only the I229C/A288C receptors, and currents were restored following application of DTT. The glycine responses were normalized to the initial glycine response (1.00, not shown), and the data was averaged. Data are expressed as the mean ± SEM. DTT, dithiothreitol; GlyR, glycine receptor; WT, wild-type.