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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: J Immunol. 2008 May 15;180(10):6923–6932. doi: 10.4049/jimmunol.180.10.6923

FIGURE 5.

FIGURE 5

IFN-γ and TNF-α secreted by human CD4+ T cells exert antiviral activity against HCV infection. A, A7 replicon cells were incubated for 24 hours in the absence (lane 1) or presence (lanes 2–11) of cytokines, neutralizing anti-cytokine antibodies, and 5% (v/v) T cell conditioned media from IL-12 + IFN-α activated T cells as indicated. Cell lysates were prepared, and Western blotting was performed using antibodies against HCV NS5A, human ISG56, and human GAPDH. B, A7 replicon cells were incubated for 24 hours in the absence (lane 1) or presence of 100 U/ml rhIFN-αA (lanes 2–3) or 5% (v/v) T cell conditioned media (lanes 4–12), in the absence or presence of 5 μg/ml anti-hIFNAR2 antibody, as indicated. Cell lysates were prepared, and Western blotting was performed using antibodies against HCV NS5A, human ISG56, and human GAPDH.