Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 May 15.

Published in final edited form as: J Immunol. 2008 May 15;180(10):6923–6932. doi: 10.4049/jimmunol.180.10.6923

IFN-γ and TNF-α secreted by CD4⁺ T cells inhibit VSV infection. THP-1 cells were infected for 16 hours with VSV-GFP. GFP expression was analyzed by flow cytometry. Data are expressed as mean +/− SEM of three replicates. (A) THP-1 cells were infected in the absence (■) or presence of 100 U/ml rhIFN-αA (▴) or increasing concentrations of rhIFN-γ ( ), rhTNF-α (◇), or rhIFN-γ + rhTNF-α (○) as indicated. (B) THP-1 cells were infected in the absence (1) or presence (2–7) of 10% (v/v) T cell conditioned media from IL-12 + IFN-α activated T cells in the absence (2) or presence of 5 μg/ml mouse IgG₁ isotype control antibody (3), 5 μg/ml anti-hIFNAR2 (4), 10 μg/ml anti-hIFNγR1 (5), 5 μg/ml anti-hTNF-α (6), or a combination of anti-hIFNγR1 and anti-hTNF-α (7).

Inline graphic — IFN-γ and TNF-α secreted by CD4⁺ T cells inhibit VSV infection. THP-1 cells were infected for 16 hours with VSV-GFP. GFP expression was analyzed by flow cytometry. Data are expressed as mean +/− SEM of three replicates. (A) THP-1 cells were infected in the absence (■) or presence of 100 U/ml rhIFN-αA (▴) or increasing concentrations of rhIFN-γ ( ), rhTNF-α (◇), or rhIFN-γ + rhTNF-α (○) as indicated. (B) THP-1 cells were infected in the absence (1) or presence (2–7) of 10% (v/v) T cell conditioned media from IL-12 + IFN-α activated T cells in the absence (2) or presence of 5 μg/ml mouse IgG₁ isotype control antibody (3), 5 μg/ml anti-hIFNAR2 (4), 10 μg/ml anti-hIFNγR1 (5), 5 μg/ml anti-hTNF-α (6), or a combination of anti-hIFNγR1 and anti-hTNF-α (7).