FIGURE 8.

Neutralization of IFN-α, IFN-β, and IFN-ω fails to inhibit the antiviral activity of human CD4⁺ T cells. THP-1 cells were infected for 16 hours with VSV-GFP. GFP expression was analyzed by flow cytometry. Data are expressed as mean +/− SEM of three replicates. (A) THP-1 cells were infected in the absence (1) or presence (2–8) of 10% (v/v) T cell conditioned media from IL-12 + IFN-α activated T cells in the absence (2) or presence of 5 μg/ml mouse IgG₁ isotype control antibody (3), 5 μg/ml anti-hIFNAR2 (4), 5 μg/ml anti-hIFN-α (5), 1700 NU/ml anti-hIFN-β (6), 5 μg/ml anti-hIFN-ω (7), or a combination of anti-hIFN-α, anti-hIFN-β, and anti-hIFN-ω (8). (B) THP-1 cells were infected in the absence or presence of 100 U/ml rhIFN-αA or 100 U/ml rhIFN-β or 10 U/ml rhIFN-ω in the absence (open bars) or presence of 5 μg/ml mouse IgG₁ isotype control antibody (hatched bars), 5 μg/ml anti-hIFNAR2 antibody (black bars), or 5 μg/ml anti-hIFN-α antibody or 1700 NU/ml anti-hIFN-β antibody or 5 μg/ml anti-hIFN-ω antibody (double hatched bars) as indicated.