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. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: Oncogene. 2007 Nov 26;27(19):2716–2727. doi: 10.1038/sj.onc.1210934

Figure 3.

Figure 3

Real time RT-PCR analysis and western blot analysis of bladder tumour-derived cell lines with (*) and without 6p22 amplification. (a) the relative expression of candidate genes CDKAL1 (black bars) and E2F3 (white bars) was determined by real-time RT-PCR. Expression levels were normalised to the control gene SDHA and relative to a pooled normal human urothelial cell (NHUC) sample. (b) ten micrograms of protein extract from a sub-panel of cell lines were analysed by western blotting with antibodies specific to E2F3, Rb, p16 or p14. The E2F3 antibody was specific for the c-terminus of the protein and detected E2F3a and b isoforms as indicated.