Table 1.
Summary of cDNA libraries and the results of expressed sequence tag (EST) analysis.
| Name | Tissue source | # Original primary clones | Fold-reduction in normalization | Average insert size | Total # sequences 1 | Total # clones1 | # Unique clones (%) | #UniESTs (%)2 | %Specific uniESTs | % of ESTs matching genome3 |
|---|---|---|---|---|---|---|---|---|---|---|
| TH1 | Hindgut and Malpighian tubules | ~107 | NA | nd | 23,236 | 14,654 | 5,537 (38%) | 2,904 (20%) | 24% | 97 |
| TL1 | Mixed larval stages | ~107 | NA | nd | 20,120 | 13,085 | 3,559 (27%) | 1,600 (12%) | 13% | 93 |
| TL2 | Normalized TL1 | NA | Beta-actin 36x | 1.5kb | 1,708 | 1,050 | 640 (61%) | 143 (14%) | 1% | 98 |
| TF1 | Fatbody full-length, normalized | 3.8 × 107 | EF1 alpha 93x | 2.4kb | 2,270 | 1,818 | 879 (45%) | 309 (17%) | 3% | 98 |
| TO1 | Ovary normalized | 5 × 106 | EF1 alpha 11.7x | 2.0kb | 1,742 | 1,082 | 727 (67%) | 323 (30%) | 2% | 67 |
| TB1 | Head (Brain) normalized | 3.6 × 106 | EF1 alpha 12x | 1.3kb | 1,448 | 855 | 633 (74%) | 251 (30%) | 2% | 94 |
| TE | Embryo | Downloaded from NCBI | 2,519 | 2,519 | 1,823 (72%) | 1,204 (48%) | 10% | 60 | ||
| EX4 | Mixed stages | Downloaded from NCBI | 8,185 | 8,185 | 4,379 (54%) | 2,121 (13%) | 17% | 95 | ||
| total | 61,228 | 43,248 | 12,351 | 72%5 | 92 | |||||
1
~70% of the clones were sequenced from both ends.
2
Expressed as a percentage of the total number of clones sequenced from the library.
3
E-value lower than 1E-50 in the BLAST search.
4
Refers to the Exelixis library (see Materials & Methods).
5
Remaining 28% of ESTs were redundant in multiple libraries.